|Application ||IHC-P, WB, E|
|Calculated MW||46837 Da|
|Antigen Region||366-397 aa|
|Other Names||Lupus La protein, La autoantigen, La ribonucleoprotein, Sjoegren syndrome type B antigen, SS-B, SSB|
|Target/Specificity||This SSB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-397 amino acids from the C-terminal region of human SSB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SSB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:3192525, PubMed:2470590). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12384597).|
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Provided below are standard protocols that you may find useful for product applications.
SSB is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(OH) elements of newly RNA polymerase III -transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. This protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome and systemic lupus erythematosus.
Bitko,V., J. Virol. 82 (16), 7977-7987 (2008)
Kotik-Kogan,O., Structure 16 (6), 852-862 (2008)
Biswas,D., Clin. Rheumatol. 27 (6), 717-722 (2008)
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