|Application ||IHC-P, WB, E|
|Other Accession||Q9D883, A1A4K8, P0DN76|
|Calculated MW||27872 Da|
|Antigen Region||55-83 aa|
|Other Names||Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP|
|Target/Specificity||This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human U2AF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||U2AF1 Antibody (Center S70) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.|
|Cellular Location||Nucleus. Nucleus speckle|
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Provided below are standard protocols that you may find useful for product applications.
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Rino,J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little,J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco,T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006)
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