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U2AF1 Antibody (Center S70)

Purified Rabbit Polyclonal Antibody (Pab)

  • IHC-P - U2AF1 Antibody (Center S70) AP2802c
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with U2AF1 antibody (Center S70), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - U2AF1 Antibody (Center S70) AP2802c
    Western blot analysis of anti-U2AF1 Antibody (Center S70) (Cat.#AP2802c) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab.
  • WB - U2AF1 Antibody (Center S70) AP2802c
    Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center S70) (Cat.#AP2802c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01081
Other Accession Q9D883, A1A4K8
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 27872 Da
Antigen Region 55-83 aa
Additional Information
Gene ID 102724594;7307
Other Names Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Target/Specificity This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids from the Central region of human U2AF1.
Dilution IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsU2AF1 Antibody (Center S70) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name U2AF1
Synonyms U2AF35, U2AFBP
Function Plays a critical role in both constitutive and enhancer- dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.
Cellular Location Nucleus. Nucleus speckle
Citations (0)

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U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.


Rino,J., Mol. Cell. Biol. 28 (9), 3045-3057 (2008) Little,J.T., J. Biol. Chem. 283 (13), 8145-8152 (2008) Pacheco,T.R., Mol. Cell. Biol. 26 (21), 8183-8190 (2006)

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$ 295.00
$ 99.00
Cat# AP2802c
(40 western blots)
Availability: In Stock
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