|Application ||WB, IHC-P, E|
|Other Accession||F1LMZ8, Q8BG32, Q2KI42|
|Calculated MW||47464 Da|
|Antigen Region||288-317 aa|
|Other Names||26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p445, PSMD11|
|Target/Specificity||This PSMD11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human PSMD11.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMD11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.|
|Cellular Location||Nucleus. Cytoplasm, cytosol|
|Tissue Location||Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate|
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Provided below are standard protocols that you may find useful for product applications.
The 26S proteasome (PSMD11) is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The protein is a non-ATPase subunit of the 19S regulator.
Saito,A., Gene 203 (2), 241-250 (1997)
Hoffman,L., FEBS Lett. 404 (2-3), 179-184 (1997)
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