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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PSMD11 Antibody (C-term)   

PSMD11 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PSMD11 Antibody (C-term) AP2803b
    Western blot analysis of PSMD11 Antibody (C-term) (Cat.#AP2803b) in 293, Jurkat cell line lysates (35ug/lane). PSMD11 (arrow) was detected using the purified Pab.
  • IHC-P - PSMD11 Antibody (C-term) AP2803b
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PSMD11 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - PSMD11 Antibody (C-term) AP2803b
    PSMD11 Antibody (C-term) (Cat.#AP2803b) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the PSMD11 antibody detected the PSMD11 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00231
Other Accession F1LMZ8, Q8BG32, Q2KI42
Reactivity Human, Mouse
Predicted Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 47464 Da
Antigen Region 288-317 aa
Additional Information
Gene ID 5717
Other Names 26S proteasome non-ATPase regulatory subunit 11, 26S proteasome regulatory subunit RPN6, 26S proteasome regulatory subunit S9, 26S proteasome regulatory subunit p445, PSMD11
Target/Specificity This PSMD11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human PSMD11.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPSMD11 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PSMD11
Function Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, PSMD11 is required for proteasome assembly. Plays a key role in increased proteasome activity in embryonic stem cells (ESCs): its high expression in ESCs promotes enhanced assembly of the 26S proteasome, followed by higher proteasome activity.
Cellular Location Nucleus. Cytoplasm, cytosol
Tissue Location Highly expressed in embryonic stem cells (ESCs). Expression decreases as ESCs differentiate
Research Areas
Citations (0)

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The 26S proteasome (PSMD11) is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The protein is a non-ATPase subunit of the 19S regulator.


Saito,A., Gene 203 (2), 241-250 (1997)
Hoffman,L., FEBS Lett. 404 (2-3), 179-184 (1997)

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Cat# AP2803b
(40 western blots)
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