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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   RUNX1 Antibody (S276)   

RUNX1 Antibody (S276)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - RUNX1 Antibody (S276) AP2805a
    All lanes : Anti-RUNX1 Antibody (S276) at 1:2000 dilution Lane 1: SW620 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - RUNX1 Antibody (S276) AP2805a
    RUNX1 Antibody (S276) (Cat. #AP2805a) western blot analysis in U-937 cell line lysates (35ug/lane).This demonstrates the RUNX1 antibody detected the RUNX1 protein (arrow).
  • IHC-P - RUNX1 Antibody (S276) AP2805a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with RUNX1 Antibody (S276) (Cat.#AP2805a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - RUNX1 Antibody (S276) AP2805a
    Confocal immunofluorescent analysis of RUNX1 Antibody (S276)(Cat#AP2805a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
  • FC - RUNX1 Antibody (S276) AP2805a
    RUNX1 Antibody (S276) (Cat. #AP2805a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01196
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 48737 Da
Antigen Region 227-255 aa
Additional Information
Gene ID 861
Other Names Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, RUNX1, AML1, CBFA2
Target/Specificity This RUNX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from human RUNX1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRUNX1 Antibody (S276) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RUNX1
Synonyms AML1, CBFA2
Function CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532).
Cellular Location Nucleus.
Tissue Location Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood
Research Areas
Citations (0)

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Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The RUNX1 protein represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.


Moosavi,S.A., Cancer Genet. Cytogenet. 189 (1), 24-28 (2009)
Zen,P.R., Cancer Genet. Cytogenet. 188 (2), 112-117 (2009)

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$ 295.00
$ 99.00
Cat# AP2805a
(40 western blots)
Availability: In Stock
Bulk Size
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