|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||48737 Da|
|Antigen Region||227-255 aa|
|Other Names||Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, RUNX1, AML1, CBFA2|
|Target/Specificity||This RUNX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from human RUNX1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Precautions||RUNX1 Antibody (S276) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B- dependent transcriptional activation. Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down- regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532).|
|Tissue Location||Expressed in all tissues examined except brain and heart. Highest levels in thymus, bone marrow and peripheral blood|
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Provided below are standard protocols that you may find useful for product applications.
Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The RUNX1 protein represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis.
Moosavi,S.A., Cancer Genet. Cytogenet. 189 (1), 24-28 (2009)
Zen,P.R., Cancer Genet. Cytogenet. 188 (2), 112-117 (2009)
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