Register or Login
All
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   RFC5 Antibody (Center R185)   

RFC5 Antibody (Center R185)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RFC5 Antibody (Center R185) AP2812c
    Western blot analysis of anti-RFC5 Antibody (Center R185) (Cat.#AP2812c) in Hela cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab.
  • WB - RFC5 Antibody (Center R185) AP2812c
    Western blot analysis of anti-RFC5 Antibody (Center R185) (Cat.#AP2812c) in rat PASMC cell line lysates (35ug/lane). RFC5(arrow) was detected using the purified Pab( Provided by Hirotaka Ata).
  • WB - RFC5 Antibody (Center R185) AP2812c
    Western blot analysis of RFC5 (arrow) using rabbit polyclonal RFC5 Antibody (Center R185) (Cat.#AP2812c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the RFC5 gene.
  • FC - RFC5 Antibody (Center R185) AP2812c
    RFC5 Antibody (Center R185) (Cat. #AP2812c) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, FC, E
Primary Accession P40937
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 38497 Da
Antigen Region 170-200 aa
Additional Information
Gene ID 5985
Other Names Replication factor C subunit 5, Activator 1 36 kDa subunit, A1 36 kDa subunit, Activator 1 subunit 5, Replication factor C 36 kDa subunit, RF-C 36 kDa subunit, RFC36, RFC5
Target/Specificity This RFC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-200 amino acids from the Central region of human RFC5.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFC5 Antibody (Center R185) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFC5
Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.
Cellular Location Nucleus.
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to
info@abgent.com, and receive a free "I Love Antibodies" mug.

Background

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. RFC5 is the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.

References

Stelzl,U., Cell 122 (6), 957-968 (2005)
Andersen,J.S., Nature 433 (7021), 77-83 (2005)

FeedBack
Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at tech@abgent.com.

$ 295.00
$ 99.00
Cat# AP2812c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
Request Quote Here

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609
USA Headquarters
(888) 735-7227 / (858) 622-0099 or (858) 875-1900

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
30% off on 2,800+ Neuroscience Antibodies. PromoCode:<span class=text-red> FLASH20
Terms & Conditions