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SMPD2 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, FC, E |
|---|---|
| Primary Accession | O60906 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47646 Da |
| Antigen Region | 9-35 aa |
| Gene ID | 6610 |
|---|---|
| Other Names | Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Lyso-PAF-PLC, Neutral sphingomyelinase, N-SMase, nSMase, SMPD2 |
| Target/Specificity | This SMPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-35 amino acids from the N-terminal region of human SMPD2. |
| Dilution | WB~~1:1000 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SMPD2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SMPD2 (HGNC:11121) |
|---|---|
| Function | Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine (PubMed:10608884). Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo (PubMed:10608884). Also acts on 1-acyl-2-lyso-sn- glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine (PubMed:10608884, PubMed:14741383). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:O70572}; Multi-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SMPD2 converts sphingomyelin to ceramide and hydrolyzes 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF.
References
Kim,H.T., J. Dermatol. Sci. 46 (2), 143-146 (2007)
Tani,M., J. Biol. Chem. 282 (13), 10047-10056 (2007)
Marchesini,N.,J. Biol. Chem. 279 (24), 25101-25111 (2004)
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