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POLG2 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - POLG2 Antibody (Center) AP2838c
    Anti-POLG2 Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - POLG2 Antibody (Center) AP2838c
    Western blot analysis of anti-POLG2 Antibody (Center) (Cat.#AP2838c) in K562 cell line lysates (35ug/lane). POLG2 (arrow) was detected using the purified Pab.
  • IHC-P - POLG2 Antibody (Center) AP2838c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with POLG2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9UHN1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54911 Da
Antigen Region 205-234 aa
Additional Information
Gene ID 11232
Other Names DNA polymerase subunit gamma-2, mitochondrial, DNA polymerase gamma accessory 55 kDa subunit, p55, Mitochondrial DNA polymerase accessory subunit, MtPolB, PolG-beta, POLG2, MTPOLB
Target/Specificity This POLG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-234 amino acids from the Central region of human POLG2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLG2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLG2
Synonyms MTPOLB
Function Mitochondrial polymerase processivity subunit. Stimulates the polymerase and exonuclease activities, and increases the processivity of the enzyme. Binds to ss-DNA.
Cellular Location Mitochondrion.
Citations (0)

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The accuracy of mitochondrial DNA (mtDNA) replication depends on the coordinated action of many nuclear-encoded proteins and on the correct balance of nucleotides within the mitochondrial matrix. mtDNA is replicated by DNA polymerase gamma, which is composed of a 140-kD catalytic subunit(POLG1) and a 55-kD accessory subunit (POLG2).


Longley,M.J., Am. J. Hum. Genet. 78 (6), 1026-1034 (2006)
Carrodeguas,J.A., J. Biol. Chem. 277 (51), 50008-50014 (2002)

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$ 295.00
Cat# AP2838c
(40 western blots)
Availability: In Stock
Bulk Size
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