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FEN1 Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2856c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP2856c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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- Backgrounds
FEN1 Antibody (Center) - Product info | |
| Application | WB, IF
|
| Primary Accession | P39748 |
| Other Accession | Q58DH8 |
| Reactivity | Human |
| Predicted | Bovine |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 42593 Da |
FEN1 Antibody (Center) - Additional info | |
| Gene ID 2237 | |
| Other Names FEN1; RAD2; Flap endonuclease 1; DNase IV; Flap structure-specific endonuclease 1; Maturation factor 1 | |
| Target/Specificity This FEN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250~280 amino acids from the Center region of human FEN1. | |
| Dilution WB~~1:50~100WB~~1:1000 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions FEN1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
FEN1 Antibody (Center) - Protein Information | |
| Name FEN1 | |
| Synonyms RAD2 | |
| Function Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double- stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA | |
| Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Mitochondrion (By similarity). Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage | |
FEN1 Antibody (Center) - Related products
AP2856c: FEN1 Antibody (Center)
DC00142: Human FEN1 cDNA Clone
FEN1 Antibody (Center) - Application data
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Western blot analysis of anti-FEN1 Antibody (Center) (Cat.#AP2856c) in HepG2 cell line lysates (35ug/lane). FEN1(arrow) was detected using the purified Pab.
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Western blot analysis of FEN1 (arrow) using rabbit polyclonal FEN1 Antibody (Center) (Cat.#AP2856c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FEN1 gene.
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Confocal immunofluorescent analysis of FEN1 Antibody (Center) (Cat. #AP2856c) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
FEN1 Antibody (Center) - Research Areas
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BACKGROUND
FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
REFERENCES
Hiraoka L.R., Harrington J.J., Gerhard D.S., Genomics 25:220-225(1995) Robins P., Pappin D.J.C., Wood R.D., Lindahl T.J. Biol. Chem. 269:28535-28538(1994) Gary R., Ludwig D.L., Cornelius H.L., MacInnes M.A.,J. Biol. Chem. 272:24522-24529(1997)
