|Application ||WB, FC, E|
|Other Accession||Q8R323, Q2TBV1|
|Calculated MW||40556 Da|
|Antigen Region||230-258 aa|
|Other Names||Replication factor C subunit 3, Activator 1 38 kDa subunit, A1 38 kDa subunit, Activator 1 subunit 3, Replication factor C 38 kDa subunit, RF-C 38 kDa subunit, RFC38, RFC3|
|Target/Specificity||This RFC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 230-258 amino acids from the C-terminal region of human RFC3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFC3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1.|
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Provided below are standard protocols that you may find useful for product applications.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa.
Rauen M., Burtelow M.A.J. Biol. Chem. 275:29767-29771(2000)
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