|Application ||WB, E|
|Calculated MW||44825 Da|
|Antigen Region||311-339 aa|
|Other Names||Sister chromatid cohesion protein DCC1, Defective in sister chromatid cohesion protein 1 homolog, DSCC1, DCC1|
|Target/Specificity||This DCC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human DCC1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DCC1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Loads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.|
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Provided below are standard protocols that you may find useful for product applications.
CHTF18 (MIM 613201), CHTF8 (MIM 613202), and DSCC1 are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176]; Bermudez et al., 2003 [PubMed 12930902]).
Murakami, T., et al. J. Biol. Chem. 285(45):34608-34615(2010)
Terret, M.E., et al. Nature 462(7270):231-234(2009)
Watrin, E., et al. Curr. Biol. 16(9):863-874(2006)
Bermudez, V.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(18):10237-10242(2003)
Merkle, C.J., et al. J. Biol. Chem. 278(32):30051-30056(2003)
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