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ENOA Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2879b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ENOA Antibody (C-term) - Product info | |
| Application | WB, FC, IF, IHC
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| Primary Accession | P06733 |
| Other Accession | Q4R5L2 |
| Reactivity | Human |
| Predicted | Monkey |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 47169 Da |
ENOA Antibody (C-term) - Additional info | |
| Gene ID 2023 | |
| Other Names ENO1; ENO1L1; MBPB1; MPB1; Alpha-enolase; 2-phospho-D-glycerate hydro-lyase; C-myc promoter-binding protein; Enolase 1; MBP-1; MPB-1; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein | |
| Target/Specificity This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405~434 amino acids from the C-terminal region of human ENO1. | |
| Dilution WB~~1:1000WB~~1:1000 FC~~1:10~50 IF~~1:10~50 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ENOA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ENOA Antibody (C-term) - Protein Information | |
| Name ENO1 | |
| Synonyms ENO1L1, MBPB1, MPB1 | |
| Function Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production | |
| Cellular Location Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line | |
| Tissue Location The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons | |
ENOA Antibody (C-term) - Related products
AP2879a: ENOA Antibody (N-term)
AP2879b: ENOA Antibody (C-term)
AP6526c: ENO1 Antibody (Center)
DC00833: Human ENO1 cDNA Clone
LY10917a: ENO1 Over-expression Lysate
BP2879a: ENOA Antibody (N-term) Blocking Peptide
BP2879b: ENOA Antibody (C-term) Blocking Peptide
BP6526a: ENO1 Antibody (N-term) Blocking Peptide
BP6526c: ENO1 Antibody (Center) Blocking Peptide
ENOA Antibody (C-term) - Application data
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ENOA Antibody (C-term) (Cat. #AP2879b) western blot analysis in Jurkat,MCF-7,U251 cell line and mouse brain tissue lysates (35ug/lane).This demonstrates the ENOA antibody detected the ENOA protein (arrow).
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ENOA Antibody (C-term) (Cat. #AP2879b) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of ENOA Antibody (C-term)(Cat#AP2879b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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ENOA Antibody (C-term) (Cat. #AP2879b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ENOA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
ENOA Antibody (C-term) - Research Areas
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BACKGROUND
ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
REFERENCES
Cappello,P., Int. J. Cancer 125 (3), 639-648 (2009) Wygrecka,M., Blood 113 (22), 5588-5598 (2009)
