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APRT Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - APRT Antibody (C-term) AP2893b
    All lanes : Anti-APRT Antibody (C-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - APRT Antibody (C-term) AP2893b
    APRT Antibody (C-term) (Cat. #AP2893b) western blot analysis in MCF-7 cell line and rat kidney tissue lysates (35ug/lane).This demonstrates the APRT antibody detected the APRT protein (arrow).
  • IHC-P - APRT Antibody (C-term) AP2893b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with APRT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07741
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 19608 Da
Antigen Region 143-170 aa
Additional Information
Gene ID 353
Other Names Adenine phosphoribosyltransferase, APRT, APRT
Target/Specificity This APRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-170 amino acids from the C-terminal region of human APRT.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAPRT Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Cellular Location Cytoplasm.
Research Areas
Citations ( 0 )


A conserved feature of APRT is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis.


Silva,C.H., et. al.,J. Biomol. Struct. Dyn. 25 (6), 589-597 (2008)
Di Pietro,V., et. al., Clin. Biochem. 40 (1-2), 73-80 (2007)

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$ 295.00
$ 99.00
Cat# AP2893b
(40 western blots)
Availability: In Stock
Bulk Size
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