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CFL1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CFL1 Antibody (N-term) AP2905a
    Western blot analysis of CFL1 Antibody (N-term) (Cat. #AP2905a) in MCF-7,Hela cell line and mouse brain tissue lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab.(2ug/ml)
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  • IHC-P - CFL1 Antibody (N-term) AP2905a
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CFL1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
  • FC - CFL1 Antibody (N-term) AP2905a
    Flow cytometric analysis of hela cells using CFL1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession P23528
Other Accession P45592, P10668, P18760, Q4R5C0, Q5E9F7, Q6B7M7
Reactivity Human, Mouse
Predicted Bovine, Monkey, Pig, Rat, Sheep
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 18502 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 1072
Other Names Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, CFL1, CFL
Target/Specificity This CFL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human CFL1.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCFL1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CFL1
Synonyms CFL
Function Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity (PubMed:11812157). In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics (PubMed:15580268). Required for the centralization of the mitotic spindle and symmetric division of zygotes (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells (PubMed:21834987). Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation (PubMed:23633677). Required for neural tube morphogenesis and neural crest cell migration (By similarity).
Cellular Location Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium {ECO:0000250|UniProtKB:P18760}. Cell projection, growth cone {ECO:0000250|UniProtKB:P18760}. Cell projection, axon {ECO:0000250|UniProtKB:P18760}. Note=Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide
Tissue Location Widely distributed in various tissues.
Research Areas
Citations ( 0 )

Background

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner.

References

Fazal,F., et.al., J. Biol. Chem. 284 (31), 21047-21056 (2009)

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Anonymous
2016-08-23 21:15:32
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Species tested
Human,Mouse,Rat
Application tested
WB
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature. 2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃ 3. Wash 5*5 min with TBST. 4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature. 5. Wash 5*5 min with TBST. 6. Incubate with ECL substrates and expose
$ 182.50
$ 70.00
Cat# AP2905a
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