|Application ||WB, IHC-P, FC, IF, E|
|Calculated MW||38779 Da|
|Antigen Region||258-285 aa|
|Other Names||BAG family molecular chaperone regulator 1, BAG-1, Bcl-2-associated athanogene 1, BAG1, HAP|
|Target/Specificity||This BAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-285 amino acids from the C-terminal region of human BAG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BAG1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Inhibits the pro-apoptotic function of PPP1R15A, and has anti-apoptotic activity. Markedly increases the anti-cell death function of BCL2 induced by various stimuli.|
|Cellular Location||Isoform 1: Nucleus. Cytoplasm. Note=Isoform 1 localizes predominantly to the nucleus Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 localizes predominantly to the cytoplasm. The cellular background in which it is expressed can influence whether it resides primarily in the cytoplasm or is also found in the nucleus. In the presence of BCL2, localizes to intracellular membranes (what appears to be the nuclear envelope and perinuclear membranes) as well as punctate cytosolic structures suggestive of mitochondria|
|Tissue Location||Isoform 4 is the most abundantly expressed isoform. It is ubiquitously expressed throughout most tissues, except the liver, colon, breast and uterine myometrium. Isoform 1 is expressed in the ovary and testis. Isoform 4 is expressed in several types of tumor cell lines, and at consistently high levels in leukemia and lymphoma cell lines. Isoform 1 is expressed in the prostate, breast and leukemia cell lines. Isoform 3 is the least abundant isoform in tumor cell lines (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
BAG1 binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms.
Hong,W., et.al., Biochem. Biophys. Res. Commun. 390 (1), 77-81 (2009)
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