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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PHGDH Antibody (N-term)   

PHGDH Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PHGDH Antibody (N-term) AP2936a
    Western blot analysis of PHGDH Antibody (N-term) (Cat. #AP2936a) in mouse brain tissue lysates (35ug/lane). PHGDH (arrow) was detected using the purified Pab.
  • WB - PHGDH Antibody (N-term) AP2936a
    Western blot analysis of PHGDH (arrow) using rabbit polyclonal PHGDH Antibody (N-term) (Cat. #AP2936a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHGDH gene (Lane 2) .
  • IHC-P - PHGDH Antibody (N-term) AP2936a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with PHGDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - PHGDH Antibody (N-term) AP2936a
    Flow cytometric analysis of hela cells using PHGDH Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43175
Other Accession A5GFY8, Q61753, Q60HD7, Q5EAD2
Reactivity Human, Mouse
Predicted Bovine, Monkey, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 56651 Da
Antigen Region 140-168 aa
Additional Information
Gene ID 26227
Other Names D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3
Target/Specificity This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 140-168 amino acids from the N-terminal region of human PHGDH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPHGDH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PGDH3
Research Areas
Citations (0)

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3-Phosphoglycerate dehydrogenase catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.


Klomp,L.W.,, Am. J. Hum. Genet. 67 (6), 1389-1399 (2000)

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Cat# AP2936a
(40 western blots)
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