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PHGDH Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 2
- PROTOCOLS
- BACKGROUND
Application 
| FC, IHC-P, WB, E |
|---|---|
| Primary Accession | O43175 |
| Other Accession | O08651, Q61753, Q60HD7, NP_006614 |
| Reactivity | Human |
| Predicted | Monkey, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 56651 Da |
| Antigen Region | 249-277 aa |
| Gene ID | 26227 |
|---|---|
| Other Names | D-3-phosphoglycerate dehydrogenase, 3-PGDH, PHGDH, PGDH3 |
| Target/Specificity | This PHGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human PHGDH. |
| Dilution | WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PHGDH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PHGDH |
|---|---|
| Synonyms | PGDH3 |
| Function | Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L- serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate. |
Provided below are standard protocols that you may find useful for product applications.
Background
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.
References
Du, H., et al. Reproduction 139(1):237-245(2010)
Burton, R.L., et al. Biochemistry 48(22):4808-4815(2009)
Kim, J.W., et al. Psychiatr. Genet. 19 (3), 161 (2009) :
Tabatabaie, L., et al. Hum. Mutat. 30(5):749-756(2009)
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