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YWHAG Antibody (N-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP2943a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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YWHAG Antibody (N-term) - Product info | |
| Application | WB, IHC, FC
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| Primary Accession | P61981 |
| Other Accession | Q6NRY9, Q6PCG0, Q6PC29, P61983, P61982, Q5F3W6, P68252 |
| Reactivity | Human, Mouse |
| Predicted | Bovine, Chicken, Rat, Xenopus, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 28303 Da |
YWHAG Antibody (N-term) - Additional info | |
| Gene ID 7532 | |
| Other Names YWHAG; 14-3-3 protein gamma; Protein kinase C inhibitor protein 1; 14-3-3 protein gamma, N-terminally processed | |
| Target/Specificity This YWHAG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70~100 amino acids from the N-terminal region of human YWHAG. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions YWHAG Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
YWHAG Antibody (N-term) - Protein Information | |
| Name YWHAG | |
| Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner | |
| Cellular Location Cytoplasm (By similarity). | |
| Tissue Location Highly expressed in brain, skeletal muscle, and heart | |
YWHAG Antibody (N-term) - Related products
AP2939c: YWHAG Antibody (Center)
AP2943a: YWHAG Antibody (N-term)
RI15775: YWHAG predesign siRNA
DC05025: Human YWHAG cDNA Clone
LY10798a: YWHAG Over-expression Lysate
BP2939c: YWHAG Antibody (Center) Blocking Peptide
BP2943a: YWHAG Antibody (N-term) Blocking Peptide
YWHAG Antibody (N-term) - Application data
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Western blot analysis of YWHAG Antibody (N-term) (Cat. #AP2943a) in A375 cell line(lane 1) and mouse cerebellum tissue(lane 2) lysates (35ug/lane). YWHAG (arrow) was detected using the purified Pab.
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Western blot analysis of YWHAG (arrow) using rabbit polyclonal YWHAG Antibody (N-term) (Cat. #AP2943a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the YWHAG gene.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with YWHAG Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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YWHAG Antibody (N-term) (Cat. #AP2943a) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
YWHAG Antibody (N-term) - Research Areas
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BACKGROUND
YWHAG belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.
REFERENCES
Jagemann,L.R., et.al., J. Biol. Chem. 283 (25), 17450-17462 (2008)
