- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
Phospho-YWHAE-pT232 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AP3001a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Phospho-YWHAE-pT232 Antibody - Product info | |
| Application | DB, WB, IHC, IF
|
| Primary Accession | P62258 |
| Reactivity | Human, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 29174 Da |
Phospho-YWHAE-pT232 Antibody - Additional info | |
| Gene ID 7531 | |
| Other Names YWHAE; 14-3-3 protein epsilon | |
| Target/Specificity This Phospho-YWHAE-T232 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T232 of human 14-3-3. | |
| Dilution DB~~1:500 WB~~1:100~500WB~~1:1000 IHC~~1:50~100 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-YWHAE-pT232 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-YWHAE-pT232 Antibody - Protein Information | |
| Name YWHAE | |
| Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner | |
| Cellular Location Cytoplasm (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV | |
Phospho-YWHAE-pT232 Antibody - Related products
AP10671b: YWHAE Antibody (C-term)
AP3000a: Phospho-YWHAE-pS58 Antibody
AP3001a: Phospho-YWHAE-pT232 Antibody
AP5837a: YWHAE Antibody (N-term)
RI15774: YWHAE predesign siRNA
DC00078: Human YWHAE cDNA Clone
LY14478a: YWHAE Over-expression Lysate
BP3000a: Phospho-14-3-3-S58 Antibody Blocking Peptide
BP3001a: Phospho-14-3-3-T232 Antibody Blocking Peptide
Phospho-YWHAE-pT232 Antibody - Application data
-
Dot blot analysis of anti-YWHAE-pT232 Pab (Cat. #AP3001a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
-
The anti-YWHAZ Pab (Cat. #AP3001a) is used in Western blot to detect YWHAZ in HL60 (left) and mouse brain (right) tissue lysates.
-
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
-
Confocal immunofluorescent analysis of Phospho-YWHAE-pT232 Antibody(Cat#AP3001a) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Phospho-YWHAE-pT232 Antibody - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The 14-3-3 family of proteins mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. It interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer.
REFERENCES
Urschel, S., et al., J. Biol. Chem. 280(17):16987-16993 (2005). Yanagi, M., et al., J. Hum. Genet. 50(4):210-216 (2005). Wang, X., et al., J. Biol. Chem. 279(47):49460-49469 (2004). Clark, K.L., et al., J. Biol. Chem. 279(19):19401-19406 (2004). Wang, X., et al., Mol. Cell. Biol. 23(17):6013-6026 (2003).
