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Phospho-H3-S10 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3004a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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Phospho-H3-S10 Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | P68431 |
| Other Accession | Q71DJ3 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15404 Da |
Phospho-H3-S10 Antibody - Additional info | |
| Gene ID 8968 | |
| Other Names HIST1H3A; H3FA; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l | |
| Target/Specificity This Phospho-H3-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human H3. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-H3-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-H3-S10 Antibody - Protein Information | |
| Name HIST1H3A | |
| Synonyms H3FA | |
| Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Phospho-H3-S10 Antibody - Related products
AP3003a: Phospho-H3-S10 Antibody
AP3004a: Phospho-H3-S10 Antibody
AP3007a: Phospho-H3-S28 Antibody
AP3115a: Phospho-HIST1H3B3-S10 Antibody
RI12466: HIST1H3A predesign siRNA
DC08693: Human HIST1H3A cDNA Clone
BP3003a: Phospho-H3-S10 Antibody Blocking Peptide
BP3004a: Phospho-H3-S10 Antibody Blocking Peptide
BP3007a: Phospho-H3-S28 Antibody Blocking Peptide
BP3115a: Phospho-HIST1H3B3-pS10 Antibody Blocking Peptide
AJ1353b: Histone H3 Antibody Acetyl (K14)
AJ1353e: Histone H3 Antibody Phospho (pT3)
AJ1357a: Histone-H3 Antibody Acetyl (K18)
AJ1357b: Histone-H3 Antibody Acetyl (K56)
AJ1359a: Histone-H3T Antibody Acetyl (K27)
Phospho-H3-S10 Antibody - Application data
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Western blot analysis of anti-Phospho-H3-pS10 Pab (Cat. #AP3004a) in HepG2 cell line lysate (35ug/lane).Phospho-H3-pS10(arrow) was detected using the purified Pab (1:64 dilution).
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Phospho-H3-S10 Antibody - Research Areas
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BACKGROUND
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
REFERENCES
Lusic, M., et al., EMBO J. 22(24):6550-6561 (2003). Deng, L., et al., Virology 289(2):312-326 (2001). Deng, L., et al., Virology 277(2):278-295 (2000). El Kharroubi, A., et al., Mol. Cell. Biol. 18(5):2535-2544 (1998). Albig, W., et al., Hum. Genet. 101(3):284-294 (1997).
