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Phospho-Bik(T33) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Phospho-Bik(T33) Antibody AP3042a
    Western blot analysis of anti-Bik Pab (Cat. #AP3042a) in mouse liver tissue lysate (35ug/lane). Bik(arrow) was detected using the purified Pab.
  • IHC-P - Phospho-Bik(T33) Antibody AP3042a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13323
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18016 Da
Additional Information
Gene ID 638
Other Names Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4, BIK, NBK
Target/Specificity This Bik Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T33 of human Bik.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Bik(T33) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BIK
Synonyms NBK
Function Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Cellular Location Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes
Research Areas
Citations (0)

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The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK.


Nikrad, M., et al., Mol. Cancer Ther. 4(3):443-449 (2005).
Dong, F., et al., Infect. Immun. 73(3):1861-1864 (2005).
Hur, J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(8):2351-2356 (2004).
Gillissen, B., et al., EMBO J. 22(14):3580-3590 (2003).
Arena, V., et al., Genes Chromosomes Cancer 38(1):91-96 (2003).

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$ 295.00
$ 99.00
Cat# AP3042a
(40 western blots)
Availability: In Stock
Bulk Size
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