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Phospho-cJun(S63) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-cJun(S63) Antibody AP3073a
    Dot blot analysis of anti-Phospho-cJun-S63 Antibody (Cat. #AP3073a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
  • WB - Phospho-cJun(S63) Antibody AP3073a
    Western blot analysis of anti-Phospho-cJun-pS63 Pab (Cat. #AP3073a) in mouse brain tissue lysate (35ug/lane). Mouse Phospho-cJun-pS63(arrow) was detected using the purified Pab.
  • IHC-P - Phospho-cJun(S63) Antibody AP3073a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P05412
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35676 Da
Additional Information
Gene ID 3725
Other Names Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN
Target/Specificity This cJun Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-cJun(S63) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name JUN
Function Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
Cellular Location Nucleus.
Research Areas
Citations (0)

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The gene for cJun is the putative transforming gene of avian sarcoma virus 17. The cJun protein is a transcription factor highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene maps to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.


Cheng, J., et al., J. Biol. Chem. 280(15):14492-14498 (2005).
Quan, T., et al., J. Biol. Chem. 280(9):8079-8085 (2005).
Bladh, L.G., et al., Mol. Pharmacol. 67(3):815-826 (2005).
DeNardo, D.G., et al., Mol. Endocrinol. 19(2):362-378 (2005).
Cheung, E., et al., Proc. Natl. Acad. Sci. U.S.A. 102(3):559-564 (2005).

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$ 295.00
$ 99.00
Cat# AP3073a
(40 western blots)
Availability: In Stock
Bulk Size
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