|Application ||DB, WB, IHC-P, E|
|Calculated MW||35676 Da|
|Other Names||Transcription factor AP-1, Activator protein 1, AP1, Proto-oncogene c-Jun, V-jun avian sarcoma virus 17 oncogene homolog, p39, JUN|
|Target/Specificity||This cJun Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-cJun(S63) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).|
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Provided below are standard protocols that you may find useful for product applications.
The gene for cJun is the putative transforming gene of avian sarcoma virus 17. The cJun protein is a transcription factor highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene maps to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
Cheng, J., et al., J. Biol. Chem. 280(15):14492-14498 (2005).
Quan, T., et al., J. Biol. Chem. 280(9):8079-8085 (2005).
Bladh, L.G., et al., Mol. Pharmacol. 67(3):815-826 (2005).
DeNardo, D.G., et al., Mol. Endocrinol. 19(2):362-378 (2005).
Cheung, E., et al., Proc. Natl. Acad. Sci. U.S.A. 102(3):559-564 (2005).
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