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Phospho-MAPKAPK2-S272 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3147a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Phospho-MAPKAPK2-S272 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P49137 |
| Other Accession | P49139, P49138 |
| Reactivity | Human |
| Predicted | Mouse, Rabbit |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 45568 Da |
Phospho-MAPKAPK2-S272 Antibody - Additional info | |
| Gene ID 9261 | |
| Other Names MAPKAPK2; MAP kinase-activated protein kinase 2 | |
| Target/Specificity This Phospho-MAPKAPK2-S272 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S272 of human MAPKAPK2. | |
| Dilution DB~~0.388888888889 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-MAPKAPK2-S272 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-MAPKAPK2-S272 Antibody - Protein Information | |
| Name MAPKAPK2 | |
| Function Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1, HNRNPA0, HSF1, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat- shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to regulate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3 | |
| Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation and subsequent activation releases the autoinhibitory helix, resulting in the export from the nucleus into the cytoplasm | |
| Tissue Location Expressed in all tissues examined. | |
Phospho-MAPKAPK2-S272 Antibody - Related products
AP3147a: Phospho-MAPKAPK2-S272 Antibody
AP7229a: MAPKAPK2 Antibody (N-term)
AP7229b: MAPKAPK2 Antibody (C-term)
RI13200: MAPKAPK2 predesign siRNA
BP3147a: Phospho-MAPKAPK2-S272 Antibody Blocking Peptide
BP7229a: MAPKAPK2 Antibody (N-term) Blocking Peptide
BP7229b: MAPKAPK2 Antibody (C-term) Blocking Peptide
BP7229d: MAPKAPK2 Antibody (S272) Blocking Peptide
AT2795a: MAPKAPK2 Antibody (monoclonal) (M01)
Phospho-MAPKAPK2-S272 Antibody - Application data
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Dot blot analysis of anti-Phospho-MAPKAPK2-S272 Antibody (Cat.#AP3147a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-MAPKAPK2-S272 Antibody - Research Areas
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BACKGROUND
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
REFERENCES
Lukas, S.M., et al., Biochemistry 43(31):9950-9960 (2004). Underwood, K.W., et al., Structure (Camb.) 11(6):627-636 (2003). Meng, W., et al., J. Biol. Chem. 277(40):37401-37405 (2002). Han, Q., et al., J. Biol. Chem. 277(50):48379-48385 (2002). Werz, O., et al., Proc. Natl. Acad. Sci. U.S.A. 97(10):5261-5266 (2000).
