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Phospho-MYT1(T495) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-MYT1(T495) Antibody AP3173a
    Dot blot analysis of anti-Phospho-MYT1-T495 Antibody (Cat. #AP3173a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
  • WB - Phospho-MYT1(T495) Antibody AP3173a
    The anti-Phospho-MYT1-T495 Antibody (Cat. #AP3173a) is used in Western blot to detect Phospho-MYT1-T495 in HL60 (left) and SK-BR-3 (right) tissue lysates.
  • IHC-P - Phospho-MYT1(T495) Antibody AP3173a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99640
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54521 Da
Additional Information
Gene ID 9088
Other Names Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1
Target/Specificity This MYT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T495 of human MYT1.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-MYT1(T495) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MYT1
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein
Research Areas
Citations ( 0 )


The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported.


Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004). Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003). Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2284-2289 (2003). Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002). Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).

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$ 295.00
$ 99.00
Cat# AP3173a
(40 western blots)
Availability: In Stock
Bulk Size
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