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Phospho-p27Kip1-S10 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3191a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 2
- Reviews
- Protocols
- Backgrounds
Phospho-p27Kip1-S10 Antibody - Product info | |
| Application | WB, IHC
|
| Primary Accession | P46527 |
| Other Accession | P46414, Q60439 |
| Reactivity | Human |
| Predicted | Hamster, Mouse |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 22073 Da |
Phospho-p27Kip1-S10 Antibody - Additional info | |
| Gene ID 1027 | |
| Other Names CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1 | |
| Target/Specificity This Phospho-p27Kip1-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human p27Kip1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-p27Kip1-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-p27Kip1-S10 Antibody - Protein Information | |
| Name CDKN1B | |
| Synonyms KIP1 | |
| Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry | |
| Cellular Location Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) | |
| Tissue Location Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney | |
Phospho-p27Kip1-S10 Antibody - Related products
AP10418a: 27Kip1-T157 Antibody
AP13302b: CDKN1B Antibody (C-term)
AP3191a: Phospho-p27Kip1-S10 Antibody
AP3192a: Phospho-p27Kip1-S178 Antibody
AP3193a: Phospho-p27Kip1-T157 Antibody
AP3194a: Phospho-p27Kip1-T187 Antibody
AP3529a: Phospho-p27Kip1-T198 Antibody
AP6269b: 27Kip1 Antibody (C-term)
RI10983: CDKN1B predesign siRNA
DC00893: Human CDKN1B cDNA Clone
LY12364a: CDKN1B Over-expression Lysate
BP10418a: 27Kip1-T157 Antibody Blocking peptide
BP3191a: Phospho-p27Kip1-S10 Antibody Blocking Peptide
BP3192a: Phospho-p27Kip1-S178 Antibody Blocking Peptide
BP3193a: Phospho-p27Kip1-T157 Antibody Blocking Peptide
BP3194a: Phospho-p27Kip1-T187 Antibody Blocking Peptide
BP3529a: Phospho-p27Kip1-T198 Antibody Blocking Peptide
BP6269b: 27Kip1 Antibody (C-term) Blocking Peptide
AT1481a: CDKN1B Antibody (monoclonal) (M01)
AJ1569c: p27/Kip1 Antibody (C-term)
AJ1569d: p27/Kip1 Antibody Phospho (pS10)
Phospho-p27Kip1-S10 Antibody - Application data
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The anti-Phospho-p27Kip1-S10 Pab (Cat. #AP9191a) is used in Western blot to detect Phospho-p27Kip1-S10 in HL60 tissue lysate
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Formalin-fixed and paraffin-embedded human skeletal muscle with Phospho-p27Kip1-S10 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Phospho-p27Kip1-S10 Antibody - Research Areas
- Le XF et al. The Role of p27Kip1 in Dasatinib-Enhanced Paclitaxel Cytotoxicity in Human Ovarian Cancer Cells. J Natl Cancer Inst. 2011 Sep 21;103(18):1403-22.
- Fang Z et al. Androgen Receptor Enhances p27 Degradation in Prostate Cancer Cells through Rapid and Selective TORC2 Activation. J Biol Chem. 2012 Jan 13;287(3):2090-8.
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BACKGROUND
p27Kip1 is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state.
REFERENCES
Kawamata, N., et al., Eur. J. Haematol. 74(5):424-429 (2005). Andreu, E.J., et al., Cancer Res. 65(8):3264-3272 (2005). Wingate, H., et al., J. Biol. Chem. 280(15):15148-15157 (2005). Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). Rassidakis, G.Z., et al., Blood 105(2):827-829 (2005).
