- CITATIONS: 1
|Application ||IHC-P, DB, E|
|Calculated MW||233175 Da|
|Other Names||Separin, Caspase-like protein ESPL1, Extra spindle poles-like 1 protein, Separase, ESPL1, ESP1, KIAA0165|
|Target/Specificity||This SEPARIN Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1126 of human SEPARIN.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-SEPARIN(S1126) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.|
|Cellular Location||Cytoplasm. Nucleus.|
Provided below are standard protocols that you may find useful for product applications.
The metaphase-to-anaphase transition is the final discrete event in duplication and separation of the genetic material of the cell. Its timing is regulated by the activation of the anaphase-promoting complex (APC). In both budding and fission yeast, the degradation of the Pds1 or Cut2 protein, respectively, is required for the onset of sister chromatid separation. Both proteins are APC substrates. Pds1 and Cut2 proteins associate with the yeast separin proteins Esp1 and Cut1, respectively, and prevent the separins from promoting chromatid separation. Pds1 and Cut2 are also called anaphase inhibitors or securins
Chestukhin, A., et al., Proc. Natl. Acad. Sci. U.S.A. 100(8):4574-4579 (2003).
Waizenegger, I., et al., Curr. Biol. 12(16):1368-1378 (2002).
Chen, F., et al., J. Biol. Chem. 277(19):16775-16781 (2002).
Hauf, S., et al., Science 293(5533):1320-1323 (2001).
Zou, H., et al., Science 285(5426):418-422 (1999).
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