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Phospho-SMAD3-S213 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3250a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Backgrounds
Phospho-SMAD3-S213 Antibody - Product info | |
| Application | IHC, WB
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| Primary Accession | P84022 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 48081 Da |
Phospho-SMAD3-S213 Antibody - Additional info | |
| Gene ID 4088 | |
| Other Names SMAD3; MADH3; Mothers against decapentaplegic homolog 3; JV15-2; SMAD family member 3 | |
| Target/Specificity This Phospho-SMAD3-S213 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3. | |
| Dilution IHC~~1:50~100 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-SMAD3-S213 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-SMAD3-S213 Antibody - Protein Information | |
| Name SMAD3 | |
| Synonyms MADH3 | |
| Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator | |
| Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta | |
Phospho-SMAD3-S213 Antibody - Related products
AP11730a: SMAD3 Antibody (S213)
AP3249a: Phospho-SMAD3-S208 Antibody
AP3250a: Phospho-SMAD3-S213 Antibody
AP6267a: SMAD3 Antibody (Center D201)
AP6267b: SMAD3 Antibody (Center N206)
RI14954: SMAD3 predesign siRNA
BP3248a: Phospho-SMAD3-S204 Antibody Blocking Peptide
BP3249a: Phospho-SMAD3-S208 Antibody Blocking Peptide
BP3250a: Phospho-SMAD3-S213 Antibody Blocking Peptide
BP6267a: SMAD3 Antibody (Center D201) Blocking Peptide
BP6267b: SMAD3 Antibody (Center N206) Blocking Peptide
BP9995a: SMAD3-S208 Antibody Blocking Peptide
Phospho-SMAD3-S213 Antibody - Application data
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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The anti-Phospho-SMAD3-S213 Pab (Cat. #AP3250a) is used in Western blot to detect Phospho-SMAD3-S213 in Ramos tissue lysate
Phospho-SMAD3-S213 Antibody - Research Areas
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BACKGROUND
SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80% of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.
REFERENCES
Imoto, S., et al., FEBS Lett. 579(13):2853-2862 (2005). Dubrovska, A., et al., Oncogene 24(14):2289-2297 (2005). Furumatsu, T., et al., J. Biol. Chem. 280(9):8343-8350 (2005). Kobayashi, T., et al., Biochem. Biophys. Res. Commun. 327(2):393-398 (2005). Kamaraju, A.K., et al., J. Biol. Chem. 280(2):1024-1036 (2005).
