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Phospho-FOS(T232) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-FOS(T232) Antibody AP3314a
    Dot blot analysis of Phospho-FOS-T232 polyclonal antibody (Cat# AP3314a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P01100
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40695 Da
Additional Information
Gene ID 2353
Other Names Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, FOS, G0S7
Target/Specificity This FOS Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T232 of human FOS.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-FOS(T232) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FOS
Synonyms G0S7
Function Nuclear phosphoprotein which forms a tight but non- covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD- binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Cellular Location Nucleus. Endoplasmic reticulum. Cytoplasm, cytosol. Note=In quiescent cells, present in very small amounts in the cytosol. Following induction of cell growth, first localizes to the endoplasmic reticulum and only later to the nucleus Localization at the endoplasmic reticulum requires dephosphorylation at Tyr-10 and Tyr-30
Research Areas
Citations (0)

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The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.


Gensch, E., et al., J. Biol. Chem. 279(37):39085-39093 (2004).
Myllykangas, S., et al., Genes Chromosomes Cancer 40(4):334-341 (2004).
Wu, M.Y., et al., World J. Gastroenterol. 10(4):476-480 (2004).
Monje, P., et al., Mol. Cell. Biol. 23(19):7030-7043 (2003).
Lavezzi, A.M., et al., Pathol. Int. 53(11):769-774 (2003).

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$ 295.00
$ 99.00
Cat# AP3314a
(40 western blots)
Availability: In Stock
Bulk Size
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