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Phospho-NR4A1(S351) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-NR4A1(S351) Antibody AP3339a
    Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody (Cat# AP3339a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22736
Other Accession P22829, P12813
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 64463 Da
Additional Information
Gene ID 3164
Other Names Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, NR4A1, GFRP1, HMR, NAK1
Target/Specificity This NR4A1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S351 of human NR4A1.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-NR4A1(S351) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NR4A1
Synonyms GFRP1, HMR, NAK1
Function Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.
Cellular Location Cytoplasm. Nucleus.
Tissue Location Fetal muscle and adult liver, brain and thyroid
Research Areas
Citations (0)

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NR4A1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis.


Lu, L., et al., J. Clin. Endocrinol. Metab. 89(8):4113-4118 (2004).
Castro-Obregon, S., et al., J. Biol. Chem. 279(17):17543-17553 (2004).
Lin, B., et al., Cell 116(4):527-540 (2004).
Choi, J.W., et al., Cancer Res. 64(1):35-39 (2004).
Ye, X., et al., Int. J. Biochem. Cell Biol. 36(1):98-113 (2004).

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$ 295.00
$ 99.00
Cat# AP3339a
(40 western blots)
Availability: In Stock
Bulk Size
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