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Phospho-HSPB1-S78 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3347a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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- Backgrounds
Phospho-HSPB1-S78 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P04792 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 22783 Da |
Phospho-HSPB1-S78 Antibody - Additional info | |
| Gene ID 3315 | |
| Other Names HSPB1; HSP27; HSP28; Heat shock protein beta-1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27 | |
| Target/Specificity This Phospho-HSPB1-S78 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S78 of human HSBP1. | |
| Dilution DB~~1:100~500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-HSPB1-S78 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-HSPB1-S78 Antibody - Protein Information | |
| Name HSPB1 | |
| Synonyms HSP27, HSP28 | |
| Function Involved in stress resistance and actin organization. | |
| Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles | |
| Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle | |
Phospho-HSPB1-S78 Antibody - Related products
AP3347a: Phospho-HSPB1-S78 Antibody
AP3431a: Phospho-HSPB1-pS83 Antibody
AP7199a: HSP27 (HSPB1) Antibody (S83)
AP7199b: HSP27 (HSPB1) Antibody (S15)
AP7199c: HSP27 (HSPB1) Antibody (S78)
AP7199e: HSP27 Antibody (Center)
RI12601: HSPB1 predesign siRNA
DC03357: Human HSPB1 cDNA Clone
LY12043a: HSPB1 Over-expression Lysate
BP3347a: Phospho-HSPB1-S78 Antibody Blocking Peptide
BP3431a: Phospho-HSPB1-pS83 Antibody Blocking Peptide
BP7199a: HSPB1 Antibody (S83) Blocking Peptide
BP7199b: HSPB1 Antibody (S15) Blocking Peptide
BP7199c: HSPB1 Antibody (S78) Blocking Peptide
BP7199d: HSPB1 Antibody (S82) Blocking Peptide
BP7199e: HSP27 Antibody (Center) Blocking Peptide
AJ1378b: HSP27 Antibody Phospho (pS15)
Phospho-HSPB1-S78 Antibody - Application data
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Dot blot analysis of Phospho-HSBP1-S78 polyclonal antibody (Cat# AP3347a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.
Phospho-HSPB1-S78 Antibody - Research Areas
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BACKGROUND
In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. HSBP1 exhibits rapid increased phosphorylation in response to various mitogens, tumor promoters (e.g. phorbol esters) and calcium ionophores, and high levels are associated with carcinoma of the breast and with endometrial adenocarcinomas. Heat shock of HeLa cell cultures, or treatment with arsenite, phorbol ester, or tumor necrosis factor, causes a rapid phosphorylation of preexisting HSBP1, with Ser82 as the major site and Ser78 the minor site of phosphorylation. HSBP1 may exert phosphorylation-activated functions linked with growth signaling pathways in unstressed cells. A homeostatic function at this level could protect cells from adverse effects of signal transduction systems which may be activated inappropriately during stress.
REFERENCES
Wano, C., et al., Exp. Cell Res. 298(2):584-592 (2004). Evgrafov, O.V., et al., Nat. Genet. 36(6):602-606 (2004). Song, H., et al., Biochem. Biophys. Res. Commun. 314(1):143-150 (2004). Chauhan, D., et al., Blood 102(9):3379-3386 (2003). Van Why, S.K., et al., J. Am. Soc. Nephrol. 14(1):98-106 (2003).
