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Phospho-ELK1-S389 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP3377a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Phospho-ELK1-S389 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P19419 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 44888 Da |
Phospho-ELK1-S389 Antibody - Additional info | |
| Gene ID 2002 | |
| Other Names ELK1; ETS domain-containing protein Elk-1 | |
| Target/Specificity This Phospho-ELK1-S389 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S389 of human ELK1. | |
| Dilution DB~~1:100~500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-ELK1-S389 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-ELK1-S389 Antibody - Protein Information | |
| Name ELK1 | |
| Function Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element | |
| Cellular Location Nucleus. | |
| Tissue Location Lung and testis. | |
Phospho-ELK1-S389 Antibody - Related products
AP3309a: Phospho-ELK1-S383 Antibody
AP3377a: Phospho-ELK1-S389 Antibody
AP3424a: Phospho-ELK1-T417 Antibody
DC08031: Human ELK1 cDNA Clone
LY11846a: ELK1 Over-expression Lysate
BP3309a: Phospho-ELK1-S383 Antibody Blocking Peptide
BP3377a: Phospho-ELK1-S389 Antibody Blocking Peptide
BP3424a: Phospho-ELK1-T417 Antibody Blocking Peptide
BP6282a: ELK1 Antibody (S383) Blocking Peptide
Phospho-ELK1-S389 Antibody - Application data
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Dot blot analysis of Phospho-ELK1-S389 Antibody (Cat. #AP3377a) and anti-ELK1 Non Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-ELK1-S389 Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.
REFERENCES
Salinas, S., et al., J. Cell Biol. 165(6):767-773 (2004). Yang, S.H., et al., Mol. Cell 12(1):63-74 (2003). Bebien, M., et al., Oncogene 22(12):1836-1847 (2003). Sharrocks, A.D., Biochem. Soc. Trans. 30(2):1-9 (2002). Murai, K., et al., Mol. Cell. Biol. 22(20):7083-7092 (2002).
