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Phospho-MAPK8-pY185 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3427a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Phospho-MAPK8-pY185 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P45983 |
| Other Accession | P49187, Q61831, P53779, Q8QHK8, P49185, Q91Y86 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Xenopus |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 48296 Da |
Phospho-MAPK8-pY185 Antibody - Additional info | |
| Gene ID 5599 | |
| Other Names MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; JNK-46; Stress-activated protein kinase 1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1 | |
| Target/Specificity This Phospho-MAPK8-pY185 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y185 of human MAPK8. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-MAPK8-pY185 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-MAPK8-pY185 Antibody - Protein Information | |
| Name MAPK8 | |
| Synonyms JNK1, PRKM8, SAPK1, SAPK1C | |
| Function Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1 In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH | |
| Cellular Location Cytoplasm. Nucleus. | |
Phospho-MAPK8-pY185 Antibody - Related products
AP10933c: MAPK8 Antibody (T183/Y185)
AP3427a: Phospho-MAPK8-pY185 Antibody
AP3437a: Phospho-MAPK8-pT183 Antibody
AP3644a: Phospho-MAPK8-pY185 Antibody
AP3752a: Phospho-JNK1- T183/Y185 Antibody
AP7227c: MAPK8 Antibody (Center)
AP7505a: MAPK8 Antibody (C-term)
RI13194: MAPK8 predesign siRNA
DC14699: Human MAPK8 cDNA Clone
BP3427a: Phospho-MAPK8-pY185 Antibody Blocking Peptide
BP3437a: Phospho-MAPK8-pT183 Antibody Blocking Peptide
BP3644a: Phospho-JNK1-pY185 Antibody Blocking Peptide
BP7227c: MAPK8 Antibody (Center) Blocking Peptide
Phospho-MAPK8-pY185 Antibody - Application data
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Dot blot analysis of anti-MAPK8-pY185 Phospho-specific Pab (Cat.#AP3427a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-MAPK8-pY185 Antibody - Research Areas
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BACKGROUND
MAPK8 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation.
REFERENCES
Deng, Y., et al., Cell 115(1):61-70 (2003). Luo, W., et al., J. Biol. Chem. 278(39):37451-37458 (2003). Ghosh, J., Biochem. Biophys. Res. Commun. 307(2):342-349 (2003). Utsugi, M., et al., J. Immunol. 171(2):628-635 (2003). Vivo, C., et al., J. Biol. Chem. 278(28):25461-25467 (2003).
