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Phospho-ATG16L-S213 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3442a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Phospho-ATG16L-S213 Antibody - Product info | |
| Application | DB
|
| Primary Accession | Q676U5 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 68265 Da |
Phospho-ATG16L-S213 Antibody - Additional info | |
| Gene ID 55054 | |
| Other Names ATG16L1; APG16L; Autophagy-related protein 16-1; APG16-like 1 | |
| Target/Specificity This Phospho-ATG16L-S213 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 198~228 amino acids surrounding S213 of human APG16L. Patent pending. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-ATG16L-S213 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-ATG16L-S213 Antibody - Protein Information | |
| Name ATG16L1 | |
| Synonyms APG16L | |
| Function Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II | |
| Cellular Location Cytoplasm (By similarity). Preautophagosomal structure membrane; Peripheral membrane protein (By similarity) Note=Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5 (By similarity) | |
Phospho-ATG16L-S213 Antibody - Related products
AM1817a: ATG16L1 Antibody (Ascites)
AP1817a: ATG16L Antibody (N-term)
AP1817c: ATG16L Antibody (C-term)
AP3366a: Phospho-ATG16L-S287 Antibody
AP3442a: Phospho-ATG16L-S213 Antibody
RI10452: ATG16L1 predesign siRNA
BP1817b: APG16L Antibody (L176) Blocking Peptide
BP1817c: APG16L Antibody (C-term) Blocking Peptide
BP1817d: APG16 Antibody Blocking Peptide
Phospho-ATG16L-S213 Antibody - Application data
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Dot blot analysis of Phospho-APG16L-S213 Antibody (Cat.#AP3442a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-ATG16L-S213 Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The APG12-APG5-APG16L complex is esential for the elongation of autophagic isolation membranes. This complex initially associates in uniform distribution with small vesicle membranes. During membrane elongation, the complex partitions, with a great concentration building on the outer side of the isolation membrane. Upon completion of the formation of the autophagosome, the APG12-APG5-APG16L dissociates from the membrane. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
REFERENCES
Zheng H., DNA Seq. 15:303-305(2004). Clark H.F., Genome Res. 13:2265-2270(2003). Ota T., Nat. Genet. 36:40-45(2004).
