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Phospho-RAF1-S494 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3460a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Phospho-RAF1-S494 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P04049 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 73052 Da |
Phospho-RAF1-S494 Antibody - Additional info | |
| Gene ID 5894 | |
| Other Names RAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; Raf-1 | |
| Target/Specificity This Phospho-RAF1-S494 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S494 of human RAF1. | |
| Dilution DB~~1:500 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-RAF1-S494 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-RAF1-S494 Antibody - Protein Information | |
| Name RAF1 | |
| Synonyms RAF | |
| Function Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation | |
| Cellular Location Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus | |
| Tissue Location In skeletal muscle, isoform 1 is more abundant than isoform 2 | |
Phospho-RAF1-S494 Antibody - Related products
AP3291a: Phospho-RAF1-S296 Antibody
AP3332a: Phospho-RAF1-S43 Antibody
AP3335a: Phospho-RAF1-S471 Antibody
AP3346a: Phospho-RAF1-T269 Antibody
AP3350a: Phospho-RAF1-pS338 Antibody
AP3443a: Phospho-RAF1-S259 Antibody
AP3445a: Phospho-RAF1-Y340 Antibody
AP3460a: Phospho-RAF1-S494 Antibody
AP3475a: Phospho-RAF1-Y341 Antibody
AP3511a: Phospho-RAF1-pS339 Antibody
AP7816a: RAF1 Antibody (N-term)
DC04669: Human RAF1 cDNA Clone
LY11633a: RAF1 Over-expression Lysate
BP3291a: Phospho-RAF1-S296 Antibody Blocking Peptide
BP3332a: Phospho-RAF1-S43 Antibody Blocking Peptide
BP3335a: Phospho-RAF1-S471 Antibody Blocking Peptide
BP3346a: Phospho-RAF1-T269 Antibody Blocking Peptide
BP3443a: Phospho-RAF1-S259 Antibody Blocking Peptide
BP3445a: Phospho-RAF1-Y340 Antibody Blocking Peptide
BP3460a: Phospho-RAF1-S494 Antibody Blocking Peptide
BP3475a: Phospho-RAF1-Y341 Antibody Blocking Peptide
BP3511a: Phospho-RAF1-pS339 Antibody Blocking Peptide
BP7816a: RAF1 Antibody (N-term) Blocking Peptide
BP7816d: RAF1 Antibody (S621) Blocking Peptide
BP7816e: RAF1 Antibody (S233) Blocking Peptide
BP7816f: RAF1 Antibody (S296) Blocking Peptide
BP7816g: RAF1 Antibody (S338) Blocking Peptide
BP7816h: RAF1 Antibody (Y340) Blocking Peptide
BP7816i: RAF1 Antibody (S471) Blocking Peptide
BP7816j: RAF1 Antibody (T269) Blocking Peptide
Phospho-RAF1-S494 Antibody - Application data
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Dot blot analysis of anti-RAF1-pS494 Phospho-specific Pab (RB13292) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-RAF1-S494 Antibody - Research Areas
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BACKGROUND
Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
REFERENCES
Dasgupta, P., et al., Mol. Cell. Biol. 24(21):9527-9541 (2004). Ku, N.O., et al., J. Cell Biol. 166(4):479-485 (2004). Gohlke, H., et al., Proteins 56(2):322-337 (2004). Akula, S.M., et al., Oncogene 23(30):5227-5241 (2004). Goetz, C.A., et al., J. Immunol. 172(8):4770-4778 (2004).
