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Phospho-AKT3-S472 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3468a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 2
- Reviews
- Protocols
- Backgrounds
Phospho-AKT3-S472 Antibody - Product info | |
| Application | DB
|
| Primary Accession | Q9Y243 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 55775 Da |
Phospho-AKT3-S472 Antibody - Additional info | |
| Gene ID 10000 | |
| Other Names AKT3; PKBG; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; RAC-PK-gamma; STK-2 | |
| Target/Specificity This Phospho-AKT3-S472 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S472 of human AKT3. | |
| Dilution DB~~0.388888888889 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-AKT3-S472 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-AKT3-S472 Antibody - Protein Information | |
| Name AKT3 | |
| Synonyms PKBG | |
| Function AKT3 is one of 3 closely related serine/threonine- protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase- dependent apoptosis | |
| Cellular Location Nucleus. Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation | |
| Tissue Location In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney | |
Phospho-AKT3-S472 Antibody - Related products
AM1849a: AKT3 Antibody (Ascites)
AP3468a: Phospho-AKT3-S472 Antibody
AP7030a: AKT3 Antibody (N-term)
AP7030c: AKT3 Antibody (Center)
AP8647b: AIK3 Antibody (C-term)
BP3468a: Phospho-AKT3-S472 Antibody Blocking Peptide
BP7030c: AKT3 Antibody (Center) Blocking Peptide
BP7030d: AKT3 Antibody (S472) Blocking Peptide
BP7030e: AKT3 Antibody (T447) Blocking Peptide
BP8647b: AIK3 Antibody (C-term) Blocking Peptide
Phospho-AKT3-S472 Antibody - Application data
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Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab (RB13331) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-AKT3-S472 Antibody - Research Areas
- Silva GB et al. Akt1 mediates purinergic-dependent NOS3 activation in thick ascending limbs.Am J Physiol Renal Physiol. 2009 Sep;297(3):F646-52. doi: 10.1152/ajprenal.00270.2009. Epub 2009 Jul 1.
- Huang W et al. Blockade of CCN6 (WISP3) Activates Growth Factor–Independent Survival and Resistance to Anoikis in Human Mammary Epithelial Cells.Cancer Res. 2010 Apr 15;70(8):3340-50.
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1).
REFERENCES
Xu, Z., et al., Biochem. Biophys. Res. Commun. 312(2):388-396 (2003). Tiwari, G., et al., Mol. Cancer Res. 1(6):475-484 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Deregibus, M.C., et al., J. Biol. Chem. 277(28):25195-25202 (2002). Brodbeck, D., et al., J. Biol. Chem. 276(31):29550-29558 (2001).
