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Phospho-EIF4E-S209 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3474a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Phospho-EIF4E-S209 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P06730 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 25097 Da |
Phospho-EIF4E-S209 Antibody - Additional info | |
| Gene ID 1977 | |
| Other Names EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein | |
| Target/Specificity This Phospho-EIF4E-S209 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S209 of human EIF4E. | |
| Dilution DB~~0.388888888889 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-EIF4E-S209 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-EIF4E-S209 Antibody - Protein Information | |
| Name EIF4E | |
| Synonyms EIF4EL1, EIF4F | |
| Function Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap | |
| Cellular Location Cytoplasm, P-body. | |
Phospho-EIF4E-S209 Antibody - Related products
AM1852a: EIF4E Antibody (Ascites)
AP1954a: EIF4E Antibody (N-term)
AP3474a: Phospho-EIF4E-S209 Antibody
RI11731: EIF4E predesign siRNA
DC03466: Human EIF4E cDNA Clone
BP3474a: Phospho-EIF4E-S209 Antibody Blocking Peptide
AJ1255b: eIF-4E Antibody (C-term)
AJ1255c: eIF-4E Antibody Phospho (pS209)
Phospho-EIF4E-S209 Antibody - Application data
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Dot blot analysis of anti-EIF4E-pS209 Phospho-specific Pab (RB13353) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-EIF4E-S209 Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs.
REFERENCES
Rychlik,W., J. Biol. Chem. 262 (22), 10434-10437 (1987) Dorfman,J., Genomics 9 (4), 785-788 (1991) Pelletier,J., Genomics 10 (4), 1079-1082 (1991) Whalen,S.G., J. Biol. Chem. 271 (20), 11831-11837 (1996)
