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Phospho-DNA-PK-T2609 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP3484a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Phospho-DNA-PK-T2609 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P78527 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 469089 Da |
Phospho-DNA-PK-T2609 Antibody - Additional info | |
| Gene ID 5591 | |
| Other Names PRKDC; HYRC; HYRC1; DNA-dependent protein kinase catalytic subunit; DNPK1; p460 | |
| Target/Specificity This Phospho-DNA-PK-T2609 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T2609 of human DNA-PK. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-DNA-PK-T2609 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-DNA-PK-T2609 Antibody - Protein Information | |
| Name PRKDC | |
| Synonyms HYRC, HYRC1 | |
| Function Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2 Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D | |
| Cellular Location Nucleus. Nucleus, nucleolus. | |
Phospho-DNA-PK-T2609 Antibody - Related products
AP3483a: Phospho-DNA-PK-S2612 Antibody
AP3484a: Phospho-DNA-PK-T2609 Antibody
RI14233: PRKDC predesign siRNA
BP3483a: Phospho-DNA-PK-S2612 Antibody Blocking Peptide
Phospho-DNA-PK-T2609 Antibody - Application data
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Dot blot analysis of anti-DNA-PK-pT2609 Pab (RB08113) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-DNA-PK-T2609 Antibody - Research Areas
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BACKGROUND
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Stem cell surface receptors and activate yeast MAPK pathway.
REFERENCES
Goudelock, D.M., et al., J. Biol. Chem. 278(32):29940-29947 (2003). Ding, Q., et al., Mol. Cell. Biol. 23(16):5836-5848 (2003). Lucero, H., et al., J. Biol. Chem. 278(24):22136-22143 (2003). Calsou, P., et al., J. Mol. Biol. 326(1):93-103 (2003). Karpova, A.Y., et al., Proc. Natl. Acad. Sci. U.S.A. 99(5):2818-2823 (2002).
