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Phospho-GIT1-pY554 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3553a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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Phospho-GIT1-pY554 Antibody - Product info | |
| Application | DB
|
| Primary Accession | Q9Y2X7 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 84341 Da |
Phospho-GIT1-pY554 Antibody - Additional info | |
| Gene ID 28964 | |
| Other Names GIT1; ARF GTPase-activating protein GIT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1 | |
| Target/Specificity This Phospho-GIT1-pY554 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 540~569 amino acids surrounding S554 of human GIT1. | |
| Dilution DB~~0.388888888889 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-GIT1-pY554 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-GIT1-pY554 Antibody - Protein Information | |
| Name GIT1 | |
| Function GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine- phosphorylated paxillin in cytoplasmic complexes | |
| Cellular Location Cytoplasm. Note=Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin | |
Phospho-GIT1-pY554 Antibody - Related products
AP3552a: Phospho-GIT1-pS375 Antibody
AP3553a: Phospho-GIT1-pY554 Antibody
AP3566a: Phospho-GIT1-pY510 Antibody
AP3683a: Phospho-GIT1-pS700 Antibody
AP3734a: Phospho-GIT1- pS388 Antibody
AP7416b: GIT1 Antibody (C-term)
AP7416c: GIT1 Antibody (Center)
AP7416d: GIT1-Y554 Antibody (C-term)
AP7416e: GIT1-S375 Antibody (Center)
BP3552a: Phospho-GIT1-pS375 Antibody Blocking Peptide
BP3553a: Phospho-GIT1-pY554 Antibody Blocking Peptide
BP3566a: Phospho-GIT1-pY510 Antibody Blocking Peptide
BP3683a: Phospho-GIT1-pS700 Antibody Blocking Peptide
BP3734b: GIT1-S388 Antibody Blocking peptide
BP7416a: GIT1 Antibody (Y510) Blocking Peptide
BP7416b: GIT1 Antibody (C-term) Blocking Peptide
Phospho-GIT1-pY554 Antibody - Application data
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Dot blot analysis of anti-Phospho-GIT1-pY554 Antibody (Cat.#AP3553a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-GIT1-pY554 Antibody - Research Areas
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BACKGROUND
GIT1 is a GTPase-activating protein for the ADP ribosylation factor family. It may serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization, increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. It sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
REFERENCES
Sato,H., Biochem. Biophys. Res. Commun. 368 (1), 157-161 (2008) Jones,N.P., Mol. Cell. Biol. 27 (16), 5790-5805 (2007) Schmalzigaug,R., Cell. Signal. 19 (8), 1733-1744 (2007) Stockton,R., Mol. Biol. Cell 18 (6), 2346-2355 (2007)
