|Application ||DB, E|
|Other Accession||Q4V8C7, Q2HJ92|
|Calculated MW||34404 Da|
|Other Names||Interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX|
|Target/Specificity||This PRKRA Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S246 of human PRKRA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-PRKRA(S246) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).|
|Cellular Location||Cytoplasm, perinuclear region. Cytoplasm.|
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Provided below are standard protocols that you may find useful for product applications.
PRKRA appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. It activates EIF2AK2 in the absence of double-stranded RNA (dsRNA).
Seibler,P., Lancet Neurol 7 (5), 380-381 (2008)
Kok,K.H., J. Biol. Chem. 282 (24), 17649-17657 (2007)
Patel,R.C., EMBO J. 17 (15), 4379-4390 (1998)
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