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Phospho-FAK1-pY576 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3597a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Phospho-FAK1-pY576 Antibody - Product info | |
| Application | DB
|
| Primary Accession | Q05397 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 119233 Da |
Phospho-FAK1-pY576 Antibody - Additional info | |
| Gene ID 5747 | |
| Other Names PTK2; FAK; FAK1; Focal adhesion kinase 1; Focal adhesion kinase-related nonkinase; Protein phosphatase 1 regulatory subunit 71; Protein-tyrosine kinase 2; p125FAK; pp125FAK | |
| Target/Specificity This Phospho-FAK1-pY576 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 565~594 amino acids surrounding Y576 of human FAK1. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-FAK1-pY576 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-FAK1-pY576 Antibody - Protein Information | |
| Name PTK2 | |
| Synonyms FAK, FAK1 | |
| Function Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation Phosphorylates SRC; this increases SRC kinase activity Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription | |
| Cellular Location Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome (By similarity). Nucleus Note=Constituent of focal adhesions. Detected at microtubules | |
| Tissue Location Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level) Ubiquitous | |
Phospho-FAK1-pY576 Antibody - Related products
AP3597a: Phospho-FAK1-pY576 Antibody
AP7702a: FAK1 Antibody (N-term)
AP8614b: PTK2 Antibody (C-term)
AP8614c: PTK2 Antibody (Center)
DC05873: Human PTK2 cDNA Clone
BP3597a: Phospho-FAK1-pY576 Antibody Blocking Peptide
BP7702a: FAK1 Antibody (N-term) Blocking Peptide
BP7702d: FAK1 Antibody (Y576) Blocking Peptide
BP8614b: PTK2 Antibody (C-term) Blocking Peptide
BP8614c: PTK2 Antibody (Center) Blocking Peptide
AJ1285c: FAK Antibody Phospho (pY397)
AJ1285d: FAK Antibody Phospho (pY576)
Phospho-FAK1-pY576 Antibody - Application data
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Dot blot analysis of anti-Phospho-FAK1-pY576 Antibody (Cat.#AP3597a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-FAK1-pY576 Antibody - Research Areas
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BACKGROUND
FAK1 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of the gene encoding FAK1 may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix.
REFERENCES
Birukova AA, (2008) Am J Respir Cell Mol Biol Jindra PT, (2008) J Immunol 180, 2357-66 Fu YN, et al. (2008)Oncogene 27, 957-65 Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7 Rikova K, et al. (2007) Cell 131, 1190-203
