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Bi-phospho-MAPK7-pT218/pY220 AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3609a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
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- Backgrounds
Bi-phospho-MAPK7-pT218/pY220 Antibody - Product info | |
| Application | DB
|
| Primary Accession | Q13164 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 88386 Da |
Bi-phospho-MAPK7-pT218/pY220 Antibody - Additional info | |
| Gene ID 5598 | |
| Other Names MAPK7; BMK1; ERK5; PRKM7; Mitogen-activated protein kinase 7; Big MAP kinase 1; Extracellular signal-regulated kinase 5 | |
| Target/Specificity This Bi-phospho-ERK5-pT218/pY220 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 206~236 amino acids surrounding phosphorylated T218 and Y220 of human ERK5. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step 2-sites phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Bi-phospho-MAPK7-pT218/pY220 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Bi-phospho-MAPK7-pT218/pY220 Antibody - Protein Information | |
| Name MAPK7 | |
| Synonyms BMK1, ERK5, PRKM7 | |
| Function Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression | |
| Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation | |
| Tissue Location Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver | |
Bi-phospho-MAPK7-pT218/pY220 Antibody - Related products
AP3608a: Phospho-MAPK7-pY220 Antibody
AP3609a: Bi-phospho-MAPK7-pT218/pY220 Antibody
AP3610a: Phospho-MAPK7-pT218 Antibody
AP7504a: MAPK7 Antibody (C-term)
AP7504b: MAPK7 Antibody (T218/Y220)
RI13193: MAPK7 predesign siRNA
BP3608a: Phospho-ERK5-pY220 Antibody Blocking Peptide
BP3609a: Phospho-ERK5-pT218/pY220 Antibody Blocking Peptide
BP3610a: Phospho-ERK5-pT218 Antibody Blocking Peptide
BP7504a: ERK5 Antibody (C-term) Blocking Peptide
Bi-phospho-MAPK7-pT218/pY220 Antibody - Application data
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Dot blot analysis of Bi-phospho-ERK5-pT218/pY220 Antibody (Cat.#AP3609a) on nitrocellulose membrane. 50ng of Bi-phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Bi-phospho-MAPK7-pT218/pY220 Antibody - Research Areas
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BACKGROUND
MEK5 and ERK5, a member of the MAP kinase subfamily of the Ser/Thr protein kinases, interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. ERK5 is activated by tyrosine and threonine phosphorylation It is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver. The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell. ERK5 is autophosphorylated on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
REFERENCES
Zhou, G., et al., J. Biol. Chem. 270(21):12665-12669 (1995). Lee, J.D., et al., Biochem. Biophys. Res. Commun. 213(2):715-724 (1995).
