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H4 Antibody ( K20[Me3] )Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3657a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
H4 Antibody ( K20[Me3] ) - Product info | |
| Application | DB, WB
|
| Primary Accession | P62805 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 11367 Da |
H4 Antibody ( K20[Me3] ) - Additional info | |
| Gene ID 8370 | |
| Other Names HIST1H4A; H4/A; H4FA; Histone H4 | |
| Target/Specificity This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic methylation-peptide between 4~33 amino acids Me3 of human hH4-K20[Me3]. | |
| Dilution DB~~1:500 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step methylation specific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions H4 Antibody ( K20[Me3] ) is for research use only and not for use in diagnostic or therapeutic procedures. | |
H4 Antibody ( K20[Me3] ) - Protein Information | |
| Name HIST1H4A | |
| Synonyms H4/A, H4FA | |
| Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
H4 Antibody ( K20[Me3] ) - Related products
AP3655a: H4 Antibody ( K20[Me1] )
AP3657a: H4 Antibody ( K20[Me3] )
AP3674a: H4 Antibody ( K20 [Me2] )
AP5660a: H4-K20 Antibody (N-term)
RI12488: HIST2H4B predesign siRNA
BP3655a: hH4-K20[Me1] Blocking Peptide
BP3656a: hH4-K20[Me1] Blocking Peptide
BP3657a: hH4-K20[Me3] Blocking Peptide
BP3674a: hH4-K20 (Methyl 2) Antibody Blocking Peptide
H4 Antibody ( K20[Me3] ) - Application data
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Dot blot analysis of anti-hH4-K20[Me3] methylation-specific Pab (Cat. #AP3657a) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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H4-K20[Me3] (Cat. #AP3657a) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).
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H4-K20[Me3] (Cat. #AP3657a) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Me3 antibody detected the Me3 protein (arrow).
H4 Antibody ( K20[Me3] ) - Research Areas
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BACKGROUND
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
REFERENCES
Yan,D., et.al., Biochem. J. 408 (1), 113-121 (2007)
