|Application ||DB, IF, E|
|Other Accession||Q62600, Q28969, P70313, P29473|
|Predicted||Bovine, Mouse, Pig, Rat|
|Calculated MW||133289 Da|
|Other Names||Nitric oxide synthase, endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII, NOS3|
|Target/Specificity||This eNos Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1177 of human eNos.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-eNos(S1177) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.|
|Cellular Location||Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity|
|Tissue Location||Platelets, placenta, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
Greif,D.M., et.al., Biochemistry 41 (52), 15845-15853 (2002)
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