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Phospho-H3-S10 AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3667a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP3667a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Phospho-H3-S10 Antibody - Product info | |
| Application | DB
|
| Primary Accession | P84243 |
| Other Accession | P61830, P02299, P08898, P02302, P02301, Q6NXT2, A5PK61, Q6PI79, P84245, P84246, Q71LE2, P84244, P84249, Q6PI20, P84247, Q5E9F8, Q27532, Q9U281, Q10453, P84233, P84228, Q71DI3, Q4QRF4, P84229, P84227, Q6LED0, P68433, P68431, P68432, Q16695 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Drosophila, C.Elegans, Mouse, Pig, Rat, Rabbit, Xenopus, Yeast, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 15328 Da |
Phospho-H3-S10 Antibody - Additional info | |
| Gene ID 3021 | |
| Other Names H3F3A; H3.3A; H3F3; Histone H3.3 | |
| Target/Specificity This Phospho-H3-S10 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between 1~30 amino acids surrounding S10 of human H3. | |
| Dilution DB~~1:500 | |
| Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-H3-S10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-H3-S10 Antibody - Protein Information | |
| Name H3F3A | |
| Synonyms H3.3A, H3F3 | |
| Function Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling | |
| Cellular Location Nucleus. Chromosome. | |
Phospho-H3-S10 Antibody - Related products
AM1151a: Phospho-Histone H3-S10 Antibody
AM1151b: Phospho-Histone H3-S10 Antibody
AM1151c: Phospho-Histone H3-S10 Antibody
AP1050a: Histone H3-MeK9 Antibody
AP1051d: Histone H3 Antibody (S10)
AP3667a: Phospho-H3-S10 Antibody
AP9934a: Histone H3-K9 Antibody
RI12390: H3F3B predesign siRNA
DC00525: Human H3F3B cDNA Clone
LY11083a: H3F3B Over-expression Lysate
BP1050a: Histone H3-MeK9 Antibody Blocking Peptide
BP1051a: Histone H3 (N-term) Blocking Peptide
BP1051d: Histone H3 Antibody (S10) Blocking Peptide
BP3667a: Phospho-H3-S10 Antibody Blocking Peptide
BP8890a: H3-S10 Antibody Blocking Peptide
BP9934a: Histone H3-K9 Antibody Blocking Peptide
Phospho-H3-S10 Antibody - Application data
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Dot blot analysis of H3 Antibody (S10) Pab (Cat. #AP3667a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-H3-S10 Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
H3 is basic nuclear protein that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
REFERENCES
Teng,H., et.al., J. Biol. Chem. 284 (39), 26368-26376 (2009) Garcia,B.A., et.al., J. Biol. Chem. 282 (10), 7641-7655 (2007)
