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Phospho-H3(S10) AntibodyPurified Rabbit Polyclonal Antibody (Pab)
United StatesOrdering Information
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|AP3667a||400 µl||No||Call||DISCONTINUED INQUIRE CLICK INQUIRE Add to cart|
|AP3667a-ev||80 µl||In Stock||Call||DISCONTINED INQUIRE CLICK INQUIRE Add to cart|
- Citations : 0
Phospho-H3(S10) Antibody - Product info
|Other Accession||P61830, P02299, P08898, P02302, P02301, Q6NXT2, A5PK61, Q6PI79, P84245, P84246, Q71LE2, P84244, P84249, Q6PI20, P84247, Q5E9F8, Q27532, Q9U281, Q10453, P84233, P84228, Q71DI3, Q4QRF4, P84229, P84227, Q6LED0, P68433, P68431, P68432, Q16695|
|Predicted||Bovine, Chicken, Drosophila, C.Elegans, Mouse, Pig, Rat, Rabbit, Xenopus, Yeast, Zebrafish|
|Calculated MW||15328 Da|
Phospho-H3(S10) Antibody - Additional info
|Gene ID 3021|
H3F3A; H3.3A; H3F3; Histone H3.3
This H3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human H3.
Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Phospho-H3(S10) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Phospho-H3(S10) Antibody - Protein Information
|Synonyms H3.3A, H3F3|
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Phospho-H3(S10) Antibody - Application data
Dot blot analysis of H3 Antibody (S10) Pab (Cat. #AP3667a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
Phospho-H3(S10) Antibody - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
H3 is basic nuclear protein that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A,H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.
Teng,H., et.al., J. Biol. Chem. 284 (39), 26368-26376 (2009)
Garcia,B.A., et.al., J. Biol. Chem. 282 (10), 7641-7655 (2007)