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Me2-H4(K20) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Me2-H4(K20) Antibody AP3674a
    Western blot analysis of lysate from K562 cell line, using H4 Antibody (K20)(Cat. #AP3674a). AP3674a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • DB - Me2-H4(K20) Antibody AP3674a
    Dot blot analysis of anti-hH4-K20(Methyl 2) methylation-specific Pab (Cat. AP3674a) on nitrocellulose membrane. 50ng of methylation-peptide or Non methylation-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
  • IHC-P - Me2-H4(K20) Antibody AP3674a
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PSMA4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - Me2-H4(K20) Antibody AP3674a
    H4 Antibody ( K20 [Me2] ) (Cat. #AP3674a) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P62805
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 11367 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names Histone H4, HIST1H4A, H4/A, H4FA
Target/Specificity This H4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from human H4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMe2-H4(K20) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H4/A, H4FA
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location Nucleus. Chromosome.
Citations (0)

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Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. The nucleosome is composed of an octamer containing a H3/H4 tetramer and two H2A/H2B dimers, surrounded by approximately 146 base pairs of DNA. A diverse and elaborate array of post-translational modifications including acetylation, phosphorylation, methylation, ubiquitination, and ADP-ribosylation occurs on the N-terminal tail domains of histones.

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$ 295.00
$ 99.00
Cat# AP3674a
(40 western blots)
Availability: In Stock
Bulk Size
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