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Phospho-CASP3-S12Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP3778a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP3778a-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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Phospho-CASP3-S12 - Product info | |
| Application | DB
|
| Primary Accession | P42574 |
| Other Accession | NP_004337.2 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 31608 Da |
Phospho-CASP3-S12 - Additional info | |
| Gene ID 836 | |
| Other Names CASP3; CPP32; Caspase-3; Apopain; Cysteine protease CPP32; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12 | |
| Target/Specificity This Phospho-CASP3-S12 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S12 of human CASP3. | |
| Dilution DB~~1:500 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Phospho-CASP3-S12 is for research use only and not for use in diagnostic or therapeutic procedures. | |
Phospho-CASP3-S12 - Protein Information | |
| Name CASP3 | |
| Synonyms CPP32 | |
| Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage | |
| Cellular Location Cytoplasm. | |
| Tissue Location Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system | |
Phospho-CASP3-S12 - Related products
AP11324b: CASP3 Antibody (C-term)
AP18692a: CASP3 Antibody (N-term S12)
AP18819c: CASP3 Antibody (Center S150)
AP18915a: CASP3 Antibody (N-termS26)
AP3725a: Cleaved-CASP3 (Asp175) Antibody
AP4985d: CASP3(Asp175) Antibody
AP7563c: Caspase-3 (CASP3) Antibody (Center)
RI10799: CASP3 predesign siRNA
DC04387: Human CASP3 cDNA Clone
BP3725a: Cleaved-CASP3 (Asp175) Antibody Blocking Peptide
BP4985d: CASP3(Asp175) Antibody Blocking Peptide
BP7563c: CASP3 Antibody (Center) Blocking Peptide
AJ1131b: Caspase-3 Antibody (Active)
Phospho-CASP3-S12 - Application data
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Dot blot analysis of Phospho-CASP3-S12 Antibody Phospho-specific Pab (Cat. #AP3778a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Phospho-CASP3-S12 - Research Areas
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BACKGROUND
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
REFERENCES
Wurstle, M.L., et al. J. Biol. Chem. 285(43):33209-33218(2010) Srikanth, C.V., et al. Science 330(6002):390-393(2010) Burke, S.P., et al. J. Biol. Chem. 285(39):30061-30068(2010) Chen, W., et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 26(7):673-674(2010) Bellini, M.F., et al. Anticancer Res. 30(7):2845-2849(2010)
