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Phospho-ATG13(S355) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • DB - Phospho-ATG13(S355) Antibody AP3834a
    Dot blot analysis of ATG13 Antibody (Phospho S355) Phospho-specific Pab (Cat. #AP3834a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
DB, E
Primary Accession O75143
Other Accession Q91YI1, NP_001136145.1, NP_055556.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 56572 Da
Additional Information
Gene ID 9776
Other Names Autophagy-related protein 13, ATG13, KIAA0652
Target/Specificity This ATG13 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S355 of human ATG13.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-ATG13(S355) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG13
Synonyms KIAA0652
Function Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
Cellular Location Cytoplasm, cytosol. Preautophagosomal structure. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane; the isolation membrane sequesters a portion of the cytoplasm resulting in autophagosome formation
Research Areas
Citations (0)
citation

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Background

Autophagy factor required for autophagosome formation. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex.

References

Ferreira, R.C., et al. Nat. Genet. 42(9):777-780(2010)
Hosokawa, N., et al. Autophagy 5(7):973-979(2009)
Mercer, C.A., et al. Autophagy 5(5):649-662(2009)
Ganley, I.G., et al. J. Biol. Chem. 284(18):12297-12305(2009)
Chan, E.Y., et al. Mol. Cell. Biol. 29(1):157-171(2009)

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$ 365.00
$ 140.00
Cat# AP3834a
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