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Phospho-CCNB3(T280) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • DB - Phospho-CCNB3(T280) Antibody AP3841a
    Dot blot analysis of CCNB3 Antibody (Phospho T280) Phospho-specific Pab (Cat. #AP3841a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WWL7
Other Accession NP_149020.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 157916 Da
Additional Information
Gene ID 85417
Other Names G2/mitotic-specific cyclin-B3, CCNB3, CYCB3
Target/Specificity This CCNB3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T280 of human CCNB3.
Dilution DB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CCNB3(T280) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CCNB3
Synonyms CYCB3
Function Cyclins are positive regulatory subunits of the cyclin- dependent kinases (CDKs), and thereby play an essential role in the control of the cell cycle, notably via their destruction during cell division. Its tissue specificity suggest that it may be required during early meiotic prophase I.
Cellular Location Nucleus.
Tissue Location Testis specific. In testis, it is expressed in developing germ cells, but not in Leydig cells. Weakly or not expressed in other tissues.
Research Areas
Citations (0)

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The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chick and Drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus. Two transcript variants encoding different isoforms have been found for this gene.


Cheng, J., et al. Science 308(5725):1149-1154(2005)
Nguyen, T.B., et al. J. Biol. Chem. 277(44):41960-41969(2002)
Hrimech, M., et al. EMBO J. 21 (14), 3918 (2002) :
Lozano, J.C., et al. Biochem. Biophys. Res. Commun. 291(2):406-413(2002)
Mouland, A.J., et al. Virology 292(2):321-330(2002)

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$ 295.00
$ 99.00
Cat# AP3841a
(40 western blots)
Availability: In Stock
Bulk Size
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