|Application ||DB, E|
|Calculated MW||17785 Da|
|Other Names||Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein, p21, Cdkn1a, Cip1, Waf1|
|Target/Specificity||This mouse p21Cip1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S78 of mouse p21Cip1.|
|Precautions||Phospho-mouse p21Cip1(S78) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (PubMed:25329316). Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (By similarity).|
|Cellular Location||Cytoplasm. Nucleus|
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The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene.
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Kim, J., et al. Cytokine 50(1):42-49(2010)
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010)
Gumina, M.R., et al. Cell Cycle 9(4):820-828(2010)
Radulovich, N., et al. Mol. Cancer 9, 24 (2010) :
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