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Phospho-HUMAN-PIK3C2B(Y228) Antibody

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - Phospho-HUMAN-PIK3C2B(Y228) Antibody AP3903b
    Western blot analysis of lysate from A431 cell line, using Phospho-HUMAN-PIK3C2B(Y228)(Cat. #AP3903b). AP3903b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug treated by EGF100ng/ml for 5 min.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00750
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 184768 Da
Additional Information
Gene ID 5287
Other Names Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit beta, PI3K-C2-beta, PtdIns-3-kinase C2 subunit beta, C2-PI3K, Phosphoinositide 3-kinase-C2-beta, PIK3C2B
Target/Specificity This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 215-250 amino acids from human.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-HUMAN-PIK3C2B(Y228) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3C2B
Function Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.
Cellular Location Microsome. Cell membrane. Cytoplasm, cytosol. Nucleus. Endoplasmic reticulum. Note=Found mostly in the microsome, but also in the plasma membrane and cytosol. Nuclear in testis
Tissue Location Expressed in columnar and transitional epithelia, mononuclear cells, and ganglion cells (at protein level). Widely expressed, with highest levels in thymus and placenta and lowest in peripheral blood, skeletal muscle and kidney.
Research Areas
Citations (0)

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Phosphorylates PtdIns and PtdIns4P with a preference for PtdIns. Does not phosphorylate PtdIns(4,5)P2. May be involved in EGF and PDGF signaling cascades.


Brown R.A.,et al.Biochem. Biophys. Res. Commun. 233:537-544(1997).
Arcaro A.,et al.J. Biol. Chem. 273:33082-33090(1998).
Gregory S.G.,et al.Nature 441:315-321(2006).
Arcaro A.,et al.Mol. Cell. Biol. 20:3817-3830(2000).
Wheeler M.,et al.Mol. Cell. Biol. 21:6660-6667(2001).

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$ 295.00
$ 99.00
Cat# AP3903b
(40 western blots)
Availability: In Stock
Bulk Size
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